|
type |
Journal Article |
authors |
Petrarulo, M.; Pellegrino, S.; Marangella, M.; Cosseddu, D.; Linari, F. |
title |
High-performance liquid chromatographic microassay for L- glutamate:glyoxylate aminotransferase activity in human liver. Application in primary hyperoxaluria type 1 |
journal |
Clin Chim Acta |
Activity |
2.6.1.4 |
ui |
94139054 |
year |
(1993) |
volume |
218 |
number |
2 |
pages |
193-200. |
| |
keywords |
Chromatography, High Pressure Liquid/*methods |
abstract |
A rapid and sensitive liquid chromatographic technique to determine L- glutamate:glyoxylate and aminotransferase (EC 2.6.1.4) activity in human liver is described. Homogenised tissue was incubated for 60 min in the presence of substrates and the 2-oxoglutarate generated was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography. The procedure allowed the detection of the enzyme activity expressed by 7.5 micrograms of liver protein, it was more sensitive and less time- consuming than the spectrophotometric procedure previously used. No significant differences were found between normal controls and patients with primary hyperoxaluria. In an 8-month-infant with primary hyperoxaluria type 1, the enzyme activity was reduced to 16% of the average control values. |
last changed |
2002/11/12 16:17 |
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