|
type |
Journal Article |
authors |
Ishida, M.; Kawashima, H.; Sato, K.; Hashiguchi, K.; Ito, H.; Enei, H.; Nakamori, S. |
title |
Factors improving L-threonine production by a three L-threonine biosynthetic genes-amplified recombinant strain of Brevibacterium lactofermentum |
journal |
Biosci Biotechnol Biochem |
Activity |
4.2.3.1 |
ui |
94264409 |
year |
(1994) |
volume |
58 |
number |
4 |
pages |
768-70. |
| |
keywords |
Brevibacterium/enzymology/genetics/*metabolism |
abstract |
When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor. |
last changed |
2002/11/12 16:17 |
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