Activities | Families | Sequences | Fold types | References | Help
B6db references: 95010756

type Journal Article
authors Mosca, M.; Cozzi, L.; Breton, J.; Speciale, C.; Okuno, E.; Schwarcz, R.; Benatti, L.
title Molecular cloning of rat kynurenine aminotransferase: identity with glutamine transaminase K
journal FEBS Lett
ui 95010756
year (1994)
volume 353
number 1
pages 21-4.
keywords Amino Acid Sequence
abstract The enzyme kynurenine aminotransferase (KAT) catalyses the conversion of L-kynurenine to kynurenic acid. A combination of polymerase chain reaction techniques and hybridization screening was used to isolate a cDNA clone encompassing the entire coding region of KAT from rat kidney. Identification of the cDNA as coding for KAT was based both on the comparison of amino acid sequences obtained from purified rat KAT and on the expression of KAT activity in COS-1 cells transfected with the cDNA. RNA blot analysis indicated that KAT mRNA is widely expressed in rat tissues. Cultured cells transfected with the cDNA for KAT also showed glutamine transaminase K activity. Based mainly on sequence data, these results demonstrate that rat kidney KAT is identical with glutamine transaminase K.
last changed 2002/11/12 16:17

B6db references