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B6db references: 95107236

type Journal Article
authors Hundle, B.; Alberti, M.; Nievelstein, V.; Beyer, P.; Kleinig, H.; Armstrong, G. A.; Burke, D. H.; Hearst, J. E.
title Functional assignment of Erwinia herbicola Eho10 carotenoid genes expressed in Escherichia coli
journal Mol Gen Genet
ui 95107236
year (1994)
volume 245
number 4
pages 406-16.
keywords Amino Acid Sequence
abstract Erwinia herbicola is a nonphotosynthetic bacterium that is yellow pigmented due to the presence of carotenoids. When the Erwinia carotenoid biosynthetic genes are expressed in Escherichia coli, this bacterium also displays a yellow phenotype. The DNA sequence of the plasmid pPL376, carrying the entire Erwinia carotenoid gene cluster, has been found to contain 12 open reading frames (ORFs). Six of the ORFs have been identified as carotenoid biosynthesis genes that code for all the enzymes required for conversion of farnesyl pyrophosphate (FPP) to zeaxanthin diglucoside via geranylgeranyl pyrophosphate, phytoene, lycopene, beta-carotene, and zeaxanthin. These enzymatic steps were assigned after disruption of each ORF by a specific mutation and analysis of the accumulated intermediates. Carotenoid intermediates were identified by the absorption spectra of the colored components and by high pressure liquid chromatographic analysis. The six carotenoid genes are arranged in at least two operons. The gene coding for beta- carotene hydroxylase is transcribed in the opposite direction from that of the other carotenoid genes and overlaps with the gene for phytoene synthase.
last changed 2003/03/17 15:05

B6db references