|
type |
Journal Article |
authors |
Tyagi, R. K.; Datta, K. |
title |
A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method |
journal |
Protein Expr Purif |
Activity |
2.6.1.43 |
ui |
95161932 |
year |
(1994) |
volume |
5 |
number |
6 |
pages |
527-33. |
| |
keywords |
Animal |
abstract |
The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat kidney mitochondria. The three-step procedure involves the use of digitonin and lubrol for mitochondrial matrix preparation and L-alanine-Sepharose 4B column chromatography followed by gel filtration on Sepharose 6B. By this procedure it is possible to obtain a highly purified enzyme preparation in a relatively short time with a 37.5% yield. |
last changed |
2002/11/12 16:17 |
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