|
type |
Journal Article |
authors |
Barroso, C.; Vega, J. M.; Gotor, C. |
title |
A new member of the cytosolic O-acetylserine(thiol)lyase gene family in Arabidopsis thaliana |
journal |
FEBS Lett |
Activity |
2.5.1.47 |
ui |
95246850 |
year |
(1995) |
volume |
363 |
number |
1-2 |
pages |
1-5. |
| |
keywords |
Amino Acid Sequence |
abstract |
A cDNA, Atcys-3A, encoding O-acetylserine-(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O- acetylserine(thiol)lyase genes. Sequence comparison and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A. |
last changed |
2003/03/17 14:53 |
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