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B6db references: 95317413

type Journal Article
authors Malherbe, P.; Alberati-Giani, D.; Kohler, C.; Cesura, A. M.
title Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain
journal FEBS Lett
ui 95317413
year (1995)
volume 367
number 2
pages 141-4.
keywords Amino Acid Sequence
abstract A soluble aminotransferase with kynurenine aminotransferase (KAT) activity has been recently isolated from rat brain. This enzyme corresponds to a cytosolic form of glutamine transaminase K (GTK). In addition to the cytosolic enzyme, a mitochondrial-associated form of this KAT/GTK also exists. In the present work we have isolated a rat brain cDNA clone encoding a KAT/GTK enzyme identical to the soluble form but carrying an additional stretch of 32 amino acids at its NH2- terminus. Several structural features of this sequence resemble those of leader peptides for mitochondrial import. Evidence that the isolated cDNA encoded for mitochondrial KAT/GTK was obtained after transfection of HEK-293 cells with the cDNA coding for this new KAT/GTK isoenzyme. In fact, a significant enrichment of both KAT and GTK enzymatic activities was found in the crude mitochondrial fraction of the transfected cells.
last changed 2002/11/12 16:17

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