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B6db references: 96099890

type Journal Article
authors Schadewaldt, P.; Hummel, W.; Wendel, U.; Adelmeyer, F.
title Enzymatic method for determination of branched-chain amino acid aminotransferase activity
journal Anal Biochem
ui 96099890
year (1995)
volume 230
number 2
pages 199-204.
keywords Animal
abstract A spectrophotometric assay for the determination of branched-chain L- amino acid aminotransferase activity is described. It is based on the transamination of L-leucine in the presence of 2-oxoglutarate yielding 4-methyl-2-oxopentanoate. The rate of formation of the branched-chain 2- oxo acid is specifically monitored in a coupled enzymatic reaction using NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei ssp. pseudoplantarum as coupling enzyme by measuring the decrease in NADH absorbance at 334 nm. Optimized assay conditions are provided as evaluated for the (iso)enzyme from rat heart.
last changed 2002/11/12 16:17

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