|
type |
Journal Article |
authors |
Awata, S.; Nakayama, K.; Sato, A.; Kawamura, M.; Suzuki, I.; Sugahara, K.; Kodama, H. |
title |
Degradation of cystathionine gamma-lyase in rat liver lysosomes: effect of leupeptin treatment |
journal |
Biochem Mol Biol Int |
Activity |
4.4.1.1 |
Family |
4.4.1.1 |
ui |
96114735 |
year |
(1995) |
volume |
35 |
number |
6 |
pages |
1183-7. |
| |
keywords |
Animal |
abstract |
Highly purified lysosomes from the livers of normal and leupeptin- treated rats were subjected to immunoblot analysis, using antibodies against rat liver cystathionine gamma-lyase. Normal lysosomes showed small amount of single band at about 40 kDa, whereas the leupeptin- treated lysosomes showed large amounts of a major band at about 40 kDa and a minor band at about 35 kDa. These observations suggest that cystathionine gamma-lyase is sequestered into lysosomes at the original subunit molecular weight and is degraded in the lysosome by way of the sequential formation of an intermediate with distinct molecular weight. |
last changed |
2008/04/02 19:23 |
|