|
type |
Journal Article |
authors |
Patton, D. A.; Volrath, S.; Ward, E. R. |
title |
Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene |
journal |
Mol Gen Genet |
Activity |
2.6.1.62 |
ui |
96262178 |
year |
(1996) |
volume |
251 |
number |
3 |
pages |
261-6. |
| |
keywords |
Amino Acids, Diamino/*genetics |
abstract |
The Arabidopsis thaliana biotin auxotroph biol was rendered prototrophic by transformation with a chimeric transgene containing the Escherichia coli bio A gene driven by a constitutive promoter. The bio A gene encodes the biotin biosynthetic enzyme 7, 8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate that bio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase. |
last changed |
2002/11/12 16:17 |
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