type	Journal Article
authors	Patton, D. A.; Volrath, S.; Ward, E. R.
title	Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene
journal	Mol Gen Genet
Activity	2.6.1.62
ui	96262178
year	(1996)
volume	251
number	3
pages	261-6.
keywords	Amino Acids, Diamino/*genetics
abstract	The Arabidopsis thaliana biotin auxotroph biol was rendered prototrophic by transformation with a chimeric transgene containing the Escherichia coli bio A gene driven by a constitutive promoter. The bio A gene encodes the biotin biosynthetic enzyme 7, 8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate that bio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.
last changed	2002/11/12 16:17