type	Journal Article
authors	Oda, T.; Funai, T.; Miura, S.
title	In vitro association with peroxisomes and conformational change of peroxisomal serine:pyruvate/alanine:glyoxylate aminotransferase in rat and human livers
journal	Biochem Biophys Res Commun
Activity	2.6.1.51
ui	97079184
year	(1996)
volume	228
number	2
pages	341-6.
keywords	Alanine Transaminase/*chemistry/*metabolism
abstract	To understand the targeting mechanisms of peroxisomal serine:pyruvate aminotransferase, in vitro import experiments were carried out using this 43 kDa peroxisomal enzyme, which was synthesized in a coupled transcription/translation system. Being different from other peroxisomal enzymes, such as acyl-CoA oxidase and urate oxidase used in previous in vitro import experiment, the soluble form of peroxisomal serine:pyruvate aminotransferase was fairly resistant to proteinase K digestion. However, the enzyme recovered in the peroxisomal fraction was proteinase K sensitive. This indicates that the association of peroxisomal serine:pyruvate aminotransferase with the peroxisomal membrane causes a marked conformational change in the structure of this protein.
last changed	2002/11/12 16:17