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B6db references: 97079184

type Journal Article
authors Oda, T.; Funai, T.; Miura, S.
title In vitro association with peroxisomes and conformational change of peroxisomal serine:pyruvate/alanine:glyoxylate aminotransferase in rat and human livers
journal Biochem Biophys Res Commun
ui 97079184
year (1996)
volume 228
number 2
pages 341-6.
keywords Alanine Transaminase/*chemistry/*metabolism
abstract To understand the targeting mechanisms of peroxisomal serine:pyruvate aminotransferase, in vitro import experiments were carried out using this 43 kDa peroxisomal enzyme, which was synthesized in a coupled transcription/translation system. Being different from other peroxisomal enzymes, such as acyl-CoA oxidase and urate oxidase used in previous in vitro import experiment, the soluble form of peroxisomal serine:pyruvate aminotransferase was fairly resistant to proteinase K digestion. However, the enzyme recovered in the peroxisomal fraction was proteinase K sensitive. This indicates that the association of peroxisomal serine:pyruvate aminotransferase with the peroxisomal membrane causes a marked conformational change in the structure of this protein.
last changed 2002/11/12 16:17

B6db references