|
type |
Journal Article |
authors |
Oda, T.; Funai, T.; Miura, S. |
title |
In vitro association with peroxisomes and conformational change of peroxisomal serine:pyruvate/alanine:glyoxylate aminotransferase in rat and human livers |
journal |
Biochem Biophys Res Commun |
Activity |
2.6.1.51 |
ui |
97079184 |
year |
(1996) |
volume |
228 |
number |
2 |
pages |
341-6. |
| |
keywords |
Alanine Transaminase/*chemistry/*metabolism |
abstract |
To understand the targeting mechanisms of peroxisomal serine:pyruvate aminotransferase, in vitro import experiments were carried out using this 43 kDa peroxisomal enzyme, which was synthesized in a coupled transcription/translation system. Being different from other peroxisomal enzymes, such as acyl-CoA oxidase and urate oxidase used in previous in vitro import experiment, the soluble form of peroxisomal serine:pyruvate aminotransferase was fairly resistant to proteinase K digestion. However, the enzyme recovered in the peroxisomal fraction was proteinase K sensitive. This indicates that the association of peroxisomal serine:pyruvate aminotransferase with the peroxisomal membrane causes a marked conformational change in the structure of this protein. |
last changed |
2002/11/12 16:17 |
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