Activities | Families | Sequences | Fold types | References | Help
B6db references: 97086323

type Journal Article
authors Pletnev, S. V.; Isupov, M. N.; Dauter, Z.; Wilson, K. S.; Faleev, N. G.; Harutyunyan, E. G.; Demidkina, T. V.
title Purification and crystals of tyrosine phenol-lyase from Erwinia herbicola
journal Biochem Mol Biol Int
ui 97086323
year (1996)
volume 38
number 1
pages 37-42.
keywords Crystallization
abstract New method of purification of tyrosine phenol-lyase from Erwinia herbicola has been developed. The enzyme obtained is homogeneous and characterised by a specific activity which is three times higher then that described earlier. Crystals of holoenzyme complexed with monovalent cations have been grown from NaCl, KCl and (NH4)2SO4 containing solutions. The crystals belong to P6(2)22 space group. They are stable to the X-ray radiation and diffract up to 2.6-3.1 A. Asymmetric unit contains one subunit of tetrameric molecule.
last changed 2002/11/12 16:17

B6db references