|
type |
Journal Article |
authors |
Topczewski, J.; Sienko, M.; Paszewski, A. |
title |
Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase |
journal |
Curr Genet |
Activity |
2.5.1.47 |
sel |
unselected |
ui |
97263786 |
year |
(1997) |
volume |
31 |
number |
4 |
pages |
348-56. |
| |
keywords |
Amino Acid Sequence |
abstract |
The cysB gene of A. nidulans was cloned by complementation of a cysB mutation. This is the first cloned eukaryotic genomic sequence coding for cysteine synthase. The gene contains one 71-bp intron and codes for a protein of 370 amino acids. Its N-terminal region has characteristic features of transit peptides, suggesting mitochondrial localisation of the enzyme. The protein shows homology with bacterial and plant cysteine synthases among which it occupies a remote phylogenetic position and apparently represents a distinct subfamily. Transcription of the cysB gene is not appreciably regulated by the concentration of methionine in the growth medium. |
last changed |
2003/11/13 10:48 |
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