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B6db references: 98432645

type Journal Article
authors Pascarella, S.; Angelaccio, S.; Contestabile, R.; Delle Fratte, S.; Di Salvo, M.; Bossa, F.
title The structure of serine hydroxymethyltransferase as modeled by homology and validated by site-directed mutagenesis
journal Protein Sci
ui 98432645
year (1998)
volume 7
number 9
pages 1976-82.
keywords Amino Acid Sequence
abstract We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the properties of a number of site-directed mutants. New active site features are also proposed for further experimental testing.
last changed 2002/11/04 17:41

B6db references