type	Journal Article
authors	Phalip, V.; Kuhn, I.; Lemoine, Y.; Jeltsch, J. M.
title	Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake
journal	Gene
Activity	2.6.1.62
ui	99267408
year	(1999)
volume	232
number	1
pages	43-51.
keywords	Amino Acids/*metabolism
abstract	An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7, 8- diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7- keto 8-aminopelargonic acid.
last changed	2002/11/12 16:17