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B6db references: 99267408

type Journal Article
authors Phalip, V.; Kuhn, I.; Lemoine, Y.; Jeltsch, J. M.
title Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake
journal Gene
ui 99267408
year (1999)
volume 232
number 1
pages 43-51.
keywords Amino Acids/*metabolism
abstract An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7, 8- diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7- keto 8-aminopelargonic acid.
last changed 2002/11/12 16:17

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