|
type |
Journal Article |
authors |
Phalip, V.; Kuhn, I.; Lemoine, Y.; Jeltsch, J. M. |
title |
Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake |
journal |
Gene |
Activity |
2.6.1.62 |
ui |
99267408 |
year |
(1999) |
volume |
232 |
number |
1 |
pages |
43-51. |
| |
keywords |
Amino Acids/*metabolism |
abstract |
An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7, 8- diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7- keto 8-aminopelargonic acid. |
last changed |
2002/11/12 16:17 |
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