|
type |
Journal Article |
authors |
Barroso, C.; Romero, L. C.; Cejudo, F. J.; Vega, J. M.; Gotor, C. |
title |
Salt-specific regulation of the cytosolic O-acetylserine(thiol)lyase gene from Arabidopsis thaliana is dependent on abscisic acid |
journal |
Plant Mol Biol |
Activity |
2.5.1.47 |
ui |
99408273 |
year |
(1999) |
volume |
40 |
number |
4 |
pages |
729-36. |
| |
keywords |
Abscisic Acid/*pharmacology |
abstract |
The expression of Atcys-3A gene coding for cytosolic O- acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis, from Arabidopsis thaliana is significantly induced by exposure to salt and heavy-metal stresses. Addition of NaCl to mature plants induced a rapid accumulation of the mRNA throughout the leaf lamina and roots, and later on in stems, being mainly restricted to vascular tissues. The salt-specific regulation of Atcys-3A was also mediated by abscisic acid (ABA) since: (1) exogenous addition of ABA to the culture medium mimicked the salt-induced plant response by raising the level of Atcys- 3A transcript, and (2) Arabidopsis mutants aba-1 and abi2-1 were not able to respond to NaCl. Our results suggest that a high rate of cysteine biosynthesis is required in Arabidopsis under salt stress necessary for a plant protection or adaptation mechanism. This hypothesis was supported by the observation that intracellular levels of cysteine and glutathione increased up to 3-fold after salt treatment. |
last changed |
2003/03/17 14:53 |
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