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B6db references: 10467009

type Journal Article
authors Sienko, M.; Paszewski, A.
title The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase: cloning and analysis
journal Curr Genet
sel selected
ui 10467009
year (1999)
volume 35
number 6
pages 638-46
keywords Amino Acid Sequence
abstract The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase, an enzyme of the main pathway of methionine synthesis, was cloned by complementation of a metG mutation. A comparison of metG genomic DNA and a cDNA copy derived from different A. nidulans strains revealed a marked DNA sequence polymorphism manifested mostly by silent point mutations. cDNA of the A. nidulans metG gene complemented the Escherichia coli metC69 mutation impairing cystathionine beta-lyase. This gene contains two introns and codes for a protein of 439 amino acids. The protein shows homology with bacterial, yeast and plant cystathionine beta-lyases, as well as with other enzymes belonging to a large family of pyridoxal 5'-phosphate binding proteins. Transcription of the metG gene is not appreciably regulated by the concentration of sulphur source in the growth medium.
last changed 2009/01/08 14:14

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