type	Journal Article
authors	Sienko, M.; Paszewski, A.
title	The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase: cloning and analysis
journal	Curr Genet
Activity	4.4.1.8
Family	4.4.1.8.c
sel	selected
ui	10467009
year	(1999)
volume	35
number	6
pages	638-46
keywords	Amino Acid Sequence
abstract	The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase, an enzyme of the main pathway of methionine synthesis, was cloned by complementation of a metG mutation. A comparison of metG genomic DNA and a cDNA copy derived from different A. nidulans strains revealed a marked DNA sequence polymorphism manifested mostly by silent point mutations. cDNA of the A. nidulans metG gene complemented the Escherichia coli metC69 mutation impairing cystathionine beta-lyase. This gene contains two introns and codes for a protein of 439 amino acids. The protein shows homology with bacterial, yeast and plant cystathionine beta-lyases, as well as with other enzymes belonging to a large family of pyridoxal 5'-phosphate binding proteins. Transcription of the metG gene is not appreciably regulated by the concentration of sulphur source in the growth medium.
last changed	2009/01/08 14:14