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B6db references: 10977898

type Journal Article
authors Strych, U.; Huang, H. C.; Krause, K. L.; Benedik, M. J.
title Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1
journal Curr Microbiol
sel selected
ui 10977898
year (2000)
volume 41
number 4
pages 290-4
keywords Alanine Racemase/*biosynthesis/genetics
abstract Alanine racemases are ubiquitous, almost uniquely prokaryotic enzymes catalyzing the racemization between l- and d-alanine. The requirement for d-alanine as a necessary component of the bacterial cell wall makes this class of enzymes a logical target for the development of novel antibiotics. In an effort to better understand the structure and mechanism of these enzymes, we have cloned the two independent alanine racemases from Pseudomonas aeruginosa, an important opportunistic bacterial pathogen of humans and animals. The dadX(PA) and alr(PA) genes have been sequenced, overexpressed, and their activity was demonstrated by complementing d-alanine auxotrophs of Escherichia coli. Both gene products were purified to electrophoretic homogeneity, the enzymes were characterized biochemically, and preliminary crystals were obtained.
last changed 2009/01/09 13:21

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