|
type |
Journal Article |
authors |
Elrod, S.L.; Jones, A.; Berka, R.M.; Cherry, J.R. |
title |
Cloning of the Aspergillus oryzae 5-aminolevulinate synthase gene and its use as a selectable marker |
journal |
Curr Genet |
Activity |
2.3.1.37 |
Family |
2.3.1.37 |
sel |
selected |
ui |
11191214 |
year |
(2000) |
volume |
38 |
number |
5 |
pages |
291-98 |
| |
keywords |
5-Aminolevulinate Synthetase/genetics/*metabolism |
abstract |
The hemA gene encoding 5-aminolevulinate synthase, the first enzyme in heme biosynthesis, was cloned from Aspergillus oryzae and evaluated as a selectable marker for the transformation of filamentous fungi. Deletion of the hemA gene resulted in a lethal phenotype that could be rescued either by the supplementation of culture media with 5-aminolevulinic acid (ALA) or by transformation with the wild-type hemA gene, but not by growth on rich media, nor by the addition of exogenous heme. Transformation of a hemA deletion strain with the hemA gene linked to a lipase expression cassette yielded ALA prototrophs expressing lipase. The hemA gene can therefore be used as a selectable marker for the transformation of A. oryzae. |
last changed |
2009/01/12 19:14 |
|