Activities | Families | Sequences | Fold types | References | Help
B6db references: 1339280.

type Journal Article
authors Lu, Y.; O'Dowd, B. F.; Orrego, H.; Israel, Y.
title Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase
journal Biochem Biophys Res Commun
sel selected
ui 1339280
year (1992)
volume 189
number 2
pages 749-58
keywords Amino Acid Sequence
abstract We have cloned and sequenced a full-length cDNA (1083 bp) encoding the human liver cystathionine-gamma-lyase enzyme (cystathionase). The human cystathionase sequence presented a substantial deletion of 132 bases (44 amino acids) compared to that reported for rat cystathionase, and of 135 bases (45 amino acids) compared to that reported for yeast cystathionase. After re-alignment for the missing nucleotides, the human cDNA sequence shows significant amino acid homology to that for the rat enzyme (85%) and the yeast enzyme (50%). A search for an undeleted cDNA, by the polymerase chain reaction, yielded a second clone which contained the missing 132 bases. Flanking nucleotides in the latter clone were identical to those in the cDNA clone containing the deletion. The two forms of human cystathionase deduced from the two cDNA clones may be derived from two different genes or may be splice variants.
last changed 2009/06/30 19:51

B6db references