|
type |
Journal Article |
authors |
Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K. |
title |
Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius |
journal |
Bioorg Med Chem Lett |
Activity |
2.6.1.100 |
Family |
2.6.1.100 |
sel |
selected |
ui |
15582417 |
year |
(2005) |
volume |
15 |
pages |
89-92 |
| |
abstract |
2-Deoxystreptamine (DOS)-containing aminoglycoside–aminocyclitol (AmAc) antibiotics represent the majority of clinically important AmAcs. Biosynthetic investigations of formation of DOS in actinomycetes are limited to the characterization of 2-deoxy-scyllo-inosose synthase, the first step enzyme of the DOS biosynthetic pathway. A gene encoding L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from the tobramycin producer Streptomyces tenebrarius was expressed heterologously in Escherichia coli. The conversions of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and scyllo-inosose to scyllo-inosamine with the activity of TbmB were determined in vitro. The results indicate that tbmB catalyzes the second step of the DOS biosynthetic pathway during the biosynthesis of 2-deoxystreptamine, a subunit of tobramycin, in S. tenebrarius. |
last changed |
2017/11/03 18:00 |
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