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B6db references: 15901718

type Journal Article
authors Manukhov, IV, Mamaeva, DV, Rastorguev, SM, Faleev, NG, Morozova, EA, Demidkina, TV, Zavilgelsky, GB.
title A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase
journal J Bacteriol
sel selected
ui 15901718
year (2005)
volume 187
number 11
pages 3889-93
abstract Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.
last changed 2009/07/01 14:09

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