|
type |
Journal Article |
authors |
Lamichhane J, Liou K, Lee HC, Kim CG, Sohng JK. |
title |
Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris |
journal |
Biotechnol Lett |
Activity |
rubn4 |
Family |
rubn4 |
sel |
selected |
ui |
16614891 |
year |
(2006) |
volume |
28 |
number |
8 |
pages |
545-53 |
| |
abstract |
ORF's for rubN6 and rubN4 have been annotated as thymidine diphosphate glucose 4-ketoreductase and thymidine diphosphate glucose 3-aminotransferase by sequence analysis of the rubradirin biosynthetic gene cluster cloned from Streptomyces achromogenes var. rubradiris NRRL 3061. Both ORFs were heterologously expressed in Escherichia coli as His-tagged fusion proteins. The functionalities of TDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferase were verified by in vitro enzyme assay, and a biosynthetic pathway for TDP-D: -rubranitrose is proposed. |
last changed |
2014/03/03 17:41 |
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