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B6db references: 16614891

type Journal Article
authors Lamichhane J, Liou K, Lee HC, Kim CG, Sohng JK.
title Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris
journal Biotechnol Lett
Activity rubn4
Family rubn4
sel selected
ui 16614891
year (2006)
volume 28
number 8
pages 545-53
abstract ORF's for rubN6 and rubN4 have been annotated as thymidine diphosphate glucose 4-ketoreductase and thymidine diphosphate glucose 3-aminotransferase by sequence analysis of the rubradirin biosynthetic gene cluster cloned from Streptomyces achromogenes var. rubradiris NRRL 3061. Both ORFs were heterologously expressed in Escherichia coli as His-tagged fusion proteins. The functionalities of TDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferase were verified by in vitro enzyme assay, and a biosynthetic pathway for TDP-D: -rubranitrose is proposed.
last changed 2014/03/03 17:41

B6db references