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B6db references: 19450582

type Journal Article
authors Lehmann T, Pollmann S
title Gene expression and characterization of a stress-induced tyrosine decarboxylase from Arabidopsis thaliana
journal FEBS Lett.
sel selected
ui 19450582
year (2009)
volume 583
number 12
pages 1895-900
keywords doi: 10.1016/j.febslet.2009.05.017
abstract Full-length tyrosine decarboxylase cDNA (TyrDC) from Arabidopsis thaliana was identified by rapid amplification of cDNA ends-PCR and isolated by RT-PCR. The TyrDC mRNA was substantially induced by drought stress and wounding, and was considerably decreased by salt stress. By using TyrDC protein fusions with green fluorescent protein, an intracellular localization to the cytoplasm was shown. Recombinant (His)(6)-TyrDC was expressed in Escherichia coli and enzymatically characterized: it exclusively catalyzed the conversion of L-tyrosine to tyramine, exhibited an optimum temperature of 50 degrees C, and an optimum pH at approximately 8.5-9. Recombinant TyrDC protein formed tetramers, as shown by blue native gel electrophoresis.
last changed 2017/11/20 14:50

B6db references