|
type |
Journal Article |
authors |
Takagi, T.; Taniguchi, T.; Yamamoto, Y.; Shibatani, T. |
title |
Molecular cloning of the L-phenylalanine transaminase gene from Paracoccus denitrificans in Escherichia coli K-12 |
journal |
Biotechnol Appl Biochem. |
Activity |
2.6.1.58 |
sel |
selected |
ui |
2054101 |
year |
(1991) |
volume |
13 |
number |
1 |
pages |
112-9 |
| |
keywords |
Animal |
abstract |
The L-phenylalanine transaminase gene of Paracoccus denitrificans was cloned by a shotgun method using the Escherichia coli K-12 mutant DG30, which lacks three distinct transaminase genes. Plasmid pPAP142 was constructed by inserting a 2.2-kb fragment carrying the transaminase gene into pUC18. Strain E. coli K-12 HB101 cells harboring the plasmid produced 20-fold to 30-fold more transaminase than wild type P. denitrificans cells. The nucleotide sequence of the 2.2-kb fragment was determined, revealing that the deduced amino acid sequence of the transaminase of P. denitrificans is similar to that of other transaminases. |
last changed |
2006/05/11 14:11 |
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