|
type |
Journal Article |
authors |
Mast YJ, Wohlleben W, Schinko E.
|
title |
Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis |
journal |
J Biotechnol |
Activity |
pgat |
Family |
pgat |
sel |
selected |
ui |
21146568 |
year |
(2011) |
volume |
155 |
number |
1 |
pages |
63-7 |
| |
abstract |
Pristinamycin I (PI), a streptogramin type B antibiotic produced by Streptomyces pristinaespiralis, contains the aproteinogenic amino acid L-phenylglycine. Recent sequence analysis led to the identification of a set of putative phenylglycine biosynthetic genes. Successive inactivation of the individual genes resulted in a loss of PI production. Production was restored by supplementation with externally added L-phenylglycine, which demonstrates that these genes are involved in phenylglycine biosynthesis and thus probably disclosing the last essential pristinamycin biosynthetic genes. Finally, a putative pathway for phenylglycine synthesis is proposed. |
last changed |
2018/01/08 12:02 |
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