type	Journal Article
authors	Hiratsuka T, Suzuki H, Kariya R, Seo T, Minami A, Oikawa H.
title	Biosynthesis of the structurally unique polycyclopropanated polyketide-nucleoside hybrid jawsamycin (FR-900848).
journal	Angew Chem Int Ed Engl.
Activity	lipo
Family	lipo
sel	selected
ui	24756819
year	(2014)
volume	53
number	21
pages	5423-6
abstract	The biosynthetic gene cluster of antifungal agent jawsamycin (FR-900848) has been identified by heterologous expression. A series of gene inactivations and in vitro and in vivo analysis of key enzymes in the biosynthetic pathway established their functions. A novel mechanism involving a radical S-adenosyl methionine (SAM) cyclopropanase collaborating with an iterative polyketide synthase is proposed for the construction of the unique polycyclopropanated backbone. Our reconstitution system sets the stage for studying the catalytic mechanism of this intriguing contiguous cyclopropanation.
last changed	2018/03/27 10:09