type	Journal Article
authors	Singh S, Peltier-Pain P, Tonelli M, Thorson JS
title	A general NMR-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways
journal	Org Lett
Activity	2.6.1.33
Family	2.6.1.33.a
sel	selected
ui	24911465
year	(2014)
volume	16
number	12
pages	3220-3
keywords	DOI: 10.1021/ol501241a
abstract	A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.
last changed	2019/06/18 13:26