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B6db references: 25184516

type Journal Article
authors Busch F, Rajendran C, Mayans O, Löffler P, Merkl R, Sterner R.
title TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases
journal Biochemistry
sel selected
ui 25184516
year (2014)
volume 53
number 38
pages 6078-83
keywords DOI: 10.1021/bi500977y
abstract The rapid increase of the number of sequenced genomes asks for the functional annotation of the encoded enzymes. We used a combined computational-structural approach to determine the function of the TrpB2 subgroup of the tryptophan synthase β chain/β chain-like TrpB1-TrpB2 family (IPR023026). The results showed that TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the l-serine dependent synthesis of tryptophan. We found a single residue being responsible for the different substrate specificities of TrpB1 and TrpB2 and confirmed this finding by mutagenesis studies and crystallographic analysis of a TrpB2 enzyme with bound O-phospho-l-serine.
last changed 2018/01/29 14:06

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