|
type |
Journal Article |
authors |
Busch F, Rajendran C, Mayans O, Löffler P, Merkl R, Sterner R. |
title |
TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases |
journal |
Biochemistry |
Activity |
4.2.1.122 |
Family |
4.2.1.122 |
sel |
selected |
ui |
25184516 |
year |
(2014) |
volume |
53 |
number |
38 |
pages |
6078-83 |
| |
keywords |
DOI: 10.1021/bi500977y |
abstract |
The rapid increase of the number of sequenced genomes asks for the functional annotation of the encoded enzymes. We used a combined computational-structural approach to determine the function of the TrpB2 subgroup of the tryptophan synthase β chain/β chain-like TrpB1-TrpB2 family (IPR023026). The results showed that TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the l-serine dependent synthesis of tryptophan. We found a single residue being responsible for the different substrate specificities of TrpB1 and TrpB2 and confirmed this finding by mutagenesis studies and crystallographic analysis of a TrpB2 enzyme with bound O-phospho-l-serine. |
last changed |
2018/01/29 14:06 |
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