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B6db references: 29520528

type Journal Article
authors Ekimova GA, Fedorov DN, Tani A, Doronina NV, Trotsenko YA
title Distribution of 1-aminocyclopropane-1-carboxylate deaminase and D-cysteine desulfhydrase genes among type species of the genus Methylobacterium
journal Antonie Van Leeuwenhoek
sel selected
ui 29520528
year (2018)
volume 111
number 10
pages 1723-1734
keywords 1-Aminocyclopropane-1-carboxylate deaminase; 16S rRNA phylogeny; D-Cysteine desulfhydrase; Methylobacterium; Phytohormones; Plant–bacteria interactions
abstract The presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase determines the ability of bacteria to increase the resistance of plants to various types of stress. The genes of ACC deaminase (acdS) and the closely related enzyme D-cysteine desulfhydrase (dcyD) were searched in type strains of various representatives of the genus Methylobacterium. Using PCR screening and in silico searching in the available complete genome sequences of type strains, the genes were found in 28 of 48 species of the genus. Phylogenetic analysis of amino acid sequences of proteins revealed two large groups of sequences of the AcdS protein and one of the DcyD protein. The distribution of these groups correlates well with the phylogenetic tree based on the sequences of the 16S rRNA genes, which apparently indicates a different evolutionary adaptation to association with plants in the representatives of these groups. For the first time for aerobic methylotrophs it was demonstrated that the gene dcyD encodes D-cysteine desulfhydrase by cloning and recombinant protein characterization.
last changed 2020/02/21 09:34

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