|
type |
Journal Article |
authors |
Barajas JF, Zargar A, Pang B, Benites VT, Gin J, Baidoo EEK, Petzold CJ, Hillson NJ, Keasling JD |
title |
Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis
|
journal |
Chembiochem |
Activity |
flvo |
Family |
flvo |
sel |
selected |
ui |
29603548 |
year |
(2018) |
volume |
19 |
number |
13 |
pages |
1391-1395 |
| |
keywords |
Biosynthesis, Polyketide, Lactam, Fluvirucin, Transferase |
abstract |
Naturally occurring lactams, such as the polyketide-derived macrolactams,
provide a diverse class of natural products that could enhance existing
chemically produced lactams. While β-amino acid loading in the fluvirucin B2
polyketide pathway has been proposed by a previously identified putative
biosynthetic gene cluster, biochemical characterization of the complete
loading enzymes has not been described. Here we elucidate the complete
biosynthetic pathway of the β-amino acid loading pathway in fluvirucin B2
biosynthesis. We demonstrate the promiscuity of the loading pathway to
utilize a range of amino acids and further illustrate the ability to
introduce non-native acyl transferases to selectively transfer β-amino acids
onto a PKS loading platform. The results presented here provide a detailed
biochemical description of β-amino acid selection and will further aid in
future efforts to develop engineered lactam-producing PKS platforms. |
last changed |
2018/10/19 13:22 |
|