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B6db references: 3018430

type Journal Article
authors Martin, C.; Cami, B.; Borne, F.; Jeenes, D.J.; Haas, D.; Patte, J.C.
title Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli
journal Mol Gen Genet
sel selected
ui 3018430
year (1986)
volume 203
number 3
pages 430-4
keywords Amino Acids, Diamino/*pharmacology
abstract The Pseudomonas aeruginosa lysA gene encoding diaminopimelate decarboxylase (DAP-decarboxylase) was cloned into a broad host range vector. This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli, where the Pseudomonas lysA gene was poorly expressed. By contrast, the E. coli lysA gene was expressed well in P. aeruginosa and subject to lysine regulation when the E. coli LysR activator protein was provided. This indicates that the mechanism of transcriptional activation for the E. coli lysA gene is effective in the heterologous host.
last changed 2009/06/08 14:26

B6db references