|
type |
Journal Article |
authors |
Jansen RS, Mandyoli L, Hughes R, Wakabayashi S, Pinkham JT, Selbach B, Guinn KM, Rubin EJ, Sacchettini JC, Rhee KY |
title |
Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis |
journal |
Nat Commun |
Activity |
2.6.1.2 |
Family |
2.6.1.2.a |
PLP Fold Type |
1 |
sel |
selected |
ui |
32327655.1 |
year |
(2020) |
volume |
11 |
number |
1 |
pages |
1960 |
| |
keywords |
DOI: 10.1038/s41467-020-15876-8 |
abstract |
Gene rv3722c of Mycobacterium tuberculosis is essential for in vitro growth, and encodes a putative pyridoxal phosphate-binding protein of unknown function. Here we use metabolomic, genetic and structural approaches to show that Rv3722c is the primary aspartate aminotransferase of M. tuberculosis, and mediates an essential but underrecognized role in metabolism: nitrogen distribution. Rv3722c deficiency leads to virulence attenuation in macrophages and mice. Our results identify aspartate biosynthesis and nitrogen distribution as potential species-selective drug targets in M. tuberculosis. |
last changed |
2020/04/25 15:43 |
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