|
type |
Journal Article |
authors |
Fujiwara, K.; Okamura-Ikeda, K.; Motokawa, Y. |
title |
Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system |
journal |
Biochem Biophys Res Commun |
Activity |
1.4.4.2 |
Family |
1.4.4.2 |
sel |
selected |
ui |
3426593 |
year |
(1987) |
volume |
149 |
number |
2 |
pages |
621-7 |
| |
keywords |
Amino Acid Oxidoreductases/*metabolism |
abstract |
A pyridoxal 5'-phosphate-containing peptide which contained 54 amino acid residues was isolated from chicken liver P-protein of the glycine cleavage system following reduction with NaB3H4, carboxymethylation, and proteolysis with lysylendopeptidase. Two peptides which comprise the two halves of the phosphopyridoxyl peptide were isolated from apo-P-protein. Sequence analysis of these three peptides provided the primary structure of the phosphopyridoxyl peptide and revealed that the cofactor is linked to Lys-35. The pyridoxal 5'-phosphate-binding site has the His-Lys(PLP)-X structure characteristic of known pyridoxal 5'-phosphate-dependent amino acid decarboxylases, tryptophan synthase, and serine hydroxymethyltransferase. |
last changed |
2009/07/07 11:52 |
|