|
type |
Journal Article |
authors |
Medina-Kauwe, L.K.; Tillakaratne, N.J.; Wu, J.Y.; Tobin, A.J. |
title |
A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells |
journal |
J Neurochem |
Activity |
2.6.1.19 |
Family |
2.6.1.19.a |
sel |
selected |
ui |
8133261 |
year |
(1994) |
volume |
62 |
number |
4 |
pages |
1267-75 |
| |
abstract |
cDNAs encoding gamma-aminobutyric acid aminotransferase (GABA-T) were isolated from a lambda ZAP rat hippocampal cDNA expression library by two independent cloning methods, immunological screening with an antimouse GABA-T antibody and plaque hybridization with a GABA-T cDNA probe derived by polymerase chain reaction. We have produced enzymatically active GABA-T from a rat brain cDNA containing the full-length GABA-T coding region. Our rat brain GABA-T cDNAs hybridize to mRNAs in brain and peripheral tissues, including liver, kidney, and testis. We have also detected GABA-T mRNA in GABAergic cells of rat cerebellar cortex by in situ hybridization. Our rat brain GABA-T probe hybridizes to Purkinje, basket, stellate, and Golgi II cells, the same GABAergic neurons previously shown to contain glutamate decarboxylase GAD65 and GAD67. |
last changed |
2009/01/08 16:01 |
|