|
type |
Journal Article |
authors |
Nishigaki, I.; Ichinose, H.; Tamai, K.; Nagatsu, T. |
title |
Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody |
journal |
Biochem J |
Activity |
4.1.1.28 |
ui |
88326241 |
year |
(1988) |
volume |
252 |
number |
2 |
pages |
331-5. |
| |
keywords |
Adrenal Medulla/enzymology |
abstract |
Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr values of brain and adrenal-medulla enzyme were both estimated to be approx. 100,000 by gel- permeation chromatography. SDS/polyacrylamide-gel electrophoresis revealed a single band with an apparent Mr of 50,000. Western immunoblot analysis showed that the antibody recognized each enzyme. With regard to substrate specificity, pH-dependence and effect of pyridoxal 5'-phosphate as a cofactor, both enzymes were similar. |
last changed |
2002/11/12 16:17 |
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