|
type |
Journal Article |
authors |
Muller, R.; Kuttler, E.; Lanz, C.; Drewke, C.; Schmidt, K. |
title |
Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15 |
journal |
FEMS Microbiol Lett |
Activity |
2.5.1.47 |
Family |
2.5.1.47 |
sel |
selected |
ui |
8867384 |
year |
(1996) |
volume |
136 |
number |
3 |
pages |
305-8 |
| |
keywords |
Amino Acid Sequence |
abstract |
The cysteine synthase gene (cysK) from Flavobacterium K3-15 was cloned and sequenced. The gene exhibits 30-50% identity to known cysteine synthases on both the DNA and the amino acid levels. The pyridoxal phosphate binding site of the enzyme is part of a conserved motif comprising seven amino acids (SIKDRIA). The lys31 residue of the flavobacterial enzyme is conserved in all known cysteine synthases. The cysK gene from Flavobacterium K3-15 was heterologously expressed and the gene product identified by immunoblotting and determination of the enzyme activity. |
last changed |
2009/02/02 10:38 |
|