|
type |
Journal Article |
authors |
Yam, C. F.; Tunnicliff, G.; Ngo, T. T.; Barbeau, A. |
title |
A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain |
journal |
Comp Biochem Physiol B |
Activity |
4.1.1.15 |
ui |
89250316 |
year |
(1977) |
volume |
58 |
number |
1 |
pages |
115-7 |
| |
keywords |
Animal |
abstract |
1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations. |
last changed |
2002/11/12 16:17 |
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