|
type |
Journal Article |
authors |
Kaneko, M.; Fujimoto, S.; Kikugawa, M.; Tamaki, N. |
title |
Irreversible inhibition of D-3-aminoisobutyrate-pyruvate aminotransferase by gabaculine |
journal |
FEBS Lett |
Activity |
2.6.1.40 |
ui |
91092395 |
year |
(1990) |
volume |
276 |
number |
1-2 |
pages |
115-8. |
| |
keywords |
4-Aminobutyrate Transaminase/antagonists & inhibitors |
abstract |
Gabaculine, 5-amino-1,3-cyclohexadienylcarboxylate, is an analogue of GABA and a potent irreversible inhibitor of GABA aminotransferase. However, D-3-aminoisobutyrate-pyruvate aminotransferase for which GABA was neither a substrate nor an inhibitor was also inactivated by gabaculine. The Ki for D-3-aminoisobutyrate-pyruvate aminotransferase was 8.3 x 10(-6) M, and the Kcat for its turnover was 0.31 min-1 at 25 degrees C. beta-Alanine protected the enzyme from inactivation by gabaculine, but GABA did so to much a lesser extent. |
last changed |
2002/11/12 16:17 |
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