|
type |
Journal Article |
authors |
Han, K. S.; Archer, J. A.; Sinskey, A. J. |
title |
The molecular structure of the Corynebacterium glutamicum threonine synthase gene |
journal |
Mol Microbiol |
Activity |
4.2.3.1 |
ui |
91171869 |
year |
(1990) |
volume |
4 |
number |
10 |
pages |
1693-702. |
| |
keywords |
Alleles |
abstract |
The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6 kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino-terminal protein sequencing, respectively. The thrC gene encodes a 54481-Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine- specific genes hom and thrB. |
last changed |
2002/11/12 16:17 |
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