|
type |
Journal Article |
authors |
Kirby, J.; Wright, F.; Flint, H.J. |
title |
A cysteine desulphurase gene from the cellulolytic rumen anaerobe Ruminococcus flavefaciens |
journal |
Biochim Biophys Acta |
Activity |
2.8.1.7 |
Family |
2.8.1.7.a |
sel |
selected |
ui |
9675295 |
year |
(1998) |
volume |
1386 |
number |
1 |
pages |
233-7 |
| |
abstract |
A gene whose predicted product shows 40-50% sequence identity with the products of nifS genes from nitrogen-fixing bacteria was found downstream from a cellulase gene in a DNA fragment from the cellulolytic rumen anaerobe, Ruminococcus flavefaciens 17. The R. flavefaciens gene product released sulphur from l-cysteine when expressed in Escherichia coli, indicating that the R. flavefaciens NifS enzyme may play a role in sulphuration, perhaps, as in nitrogen-fixing bacteria, supplying sulphur to FeS proteins. Sequences hybridising with the R. flavefaciens 17 nifS-like gene were also detected in R. flavefaciens 007 and in R. albus SY3.
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last changed |
2009/05/19 18:01 |
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