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B6db references: 9846749

type Journal Article
authors Gu, W.; Song, J.; Bonner, C.A.; Xie, G.; Jensen, R.A.
title PhhC is an essential aminotransferase for aromatic amino acid catabolism in Pseudomonas aeruginosa
journal Microbiology
sel selected
ui 9846749
year (1998)
volume 144
number 11
pages 3127-34
abstract The phhC gene of Pseudomonas aeruginosa encodes a protein which is a member of the Family I aminotransferases. At high expression levels in the heterologous Escherichia coli system, PhhC can compensate for the absence of AspC (which functions in L-aspartate biosynthesis) and TyrB (which functions in aromatic biosynthesis). In the native organism, PhhC is essential for catabolism of either L-tyrosine or L-phenylalanine, as demonstrated by gene inactivation. This catabolic function of PhhC is consistent with its inclusion as the distal gene in the inducible phenylalanine hydroxylase operon. The presence of PhhC for catabolism of aromatic amino acids is required in spite of an existing multiplicity of other P. aeruginosa aminotransferases having a similar pattern of broad substrate specificity in vitro. This implies a spatial orientation of PhhC that effectively specializes it for aromatic amino acid catabolism.
last changed 2009/01/08 14:58

B6db references