|
type |
Journal Article |
authors |
Trobacher CP, Clark SM, Bozzo GG, Mullen RT, DeEll JR, Shelp BJ |
title |
Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases. |
journal |
Postharvest Biol Technol |
Activity |
2.6.1.96 |
Family |
2.6.1.96 |
sel |
selected |
ui |
NOTnotInPMed |
year |
(2012) |
volume |
75 |
pages |
106-113 |
| |
keywords |
10.1016/j.postharvbio.2012.08.005 |
abstract |
gamma-Aminobutyrate (GABA) accumulates in apple fruit during controlled atmosphere storage. Here, we
demonstrated that GABA levels declined markedly in apples (Malus × domestica Borkh. cv. Empire) within
3 h after transfer from controlled atmosphere storage (3 ◦C, 2.5 kPa O2, 2.5 kPa CO2) to ambient conditions.
Also, we identified two genes encoding apple fruit GABA transaminase (GABA-T), the enzyme responsible
for the catabolism of GABA to succinic semialdehyde. The deduced amino acid sequences of the two MdGABA-T enzymes were 93% identical to each other, and 74–83% identical to known Arabidopsis
and tomato GABA-Ts. Transient expression of the individual full-length proteins fused to the green fluorescent protein in tobacco suspension-cultured cells revealed that MdGABA-T1 and MdGABA-T2 were
localized to mitochondria. Removal of the N-terminal targeting presequences yielded good recovery of
the soluble recombinant proteins in Escherichia coli when they were co-expressed with the GroES/EL
molecular chaperone complex. Continuous monitoring of recombinant GABA-T activity via a bacterial
NADP+-dependent succinic semialdehyde dehydrogenase-linked assay established that the two GABATs
in apple fruit, like the mitochondrial GABA-T in Arabidopsis and the mitochondrial, plastidial and cytosolic GABA-Ts in tomato, utilized pyruvate and glyoxylate, but not 2-oxoglutarate. Thus, the substrate specificity of the two apple fruit GABA-Ts was similar to that for the GABA-Ts in Arabidopsis and tomato. However, the existence of two GABA-Ts in the mitochondria of apple fruit differed from the scenarios found in the other two species, providing yet another variation on the subcellular distribution of GABA-Ts in plant cells. |
last changed |
2019/01/08 09:25 |
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