|
type |
Journal Article |
authors |
Aberhart, D. J. |
title |
Separation by high-performance liquid chromatography of alpha- and beta-amino acids: application to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase |
journal |
Anal Biochem |
Activity |
5.4.3.2 |
ui |
Splcaaa |
year |
(1988) |
volume |
169 |
number |
2 |
pages |
350-5 |
| |
keywords |
Amino Acid Isomerases/*analysis |
abstract |
Several pairs of alpha- and beta-amino acids were well separated as the ortho-phthalaldehyde derivatives by reversed phase HPLC. These included alpha- and beta-lysine, leucine, tyrosine, serine, aminoisobutyric acid, and beta-hydroxyvaline and its positional isomer. The separation was applicable to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase. However, for unknown reasons, no leucine 2,3-aminomutase activity could be found in rat liver homogenate. |
last changed |
2002/11/12 16:17 |
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