|
type |
Journal Article |
authors |
Wilson, B.A.; Bantia, S.; Salituro, G.M; Reeve, A.M.; Townsend, C.A. |
title |
Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine. |
journal |
J. Am. Chem. Soc. |
Activity |
5.1.1.14 |
sel |
unselected |
ui |
thnocarth |
year |
(1988) |
volume |
110 |
number |
24 |
pages |
8238-9. |
| |
abstract |
A partially purified cell-free system was obtained from Nocardia uniformis supsp. tsuyamanensis (ATCC 21806) which exhibits 2 enzymic activities involved in the late stages of nocardicin A biosynthesis, a monocyclic b-lactam antibiotic. The first, a 3-amino-3-carboxypropyl transferase, catalyzes the formation of isonocardicin A from S-adenosylmethionine and nocardicin E, and the second, an epimerase, inverts the C-9' position of isonocardicin A from the L-configuration to the D-configuration to afford nocardicin A. These findings and the earlier demonstration that nocardicin G, the simplest of the 7 known nocardicins, is incorporated intact into nocardicin A, allows a complete biogenetic pathway originating in L-serine and L-(p-hydroxyphenyl)glycine to be proposed.
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last changed |
2005/05/02 18:50 |
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