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B6db references: edta

type Journal Article
authors McCullough, W.G.; Piligian, J.T.; Daniel, I.J.
title Enzymatic decarboxylation of three aminobenzoates.
journal J. Am. Chem. Soc.
sel selected
ui Edta
year (1957)
volume 79
pages 628-630
abstract From E. coli 0111 : B4 cell-free enzyme preparations have been obtained which decarboxylate p-aminobenzoic acid and anthranilic acid to aniline. These preparations do not metabolize m-aminobenzoate. Dialysis of the enzyme preparations against 0.2 M acetate buffers at pH 5.0 results in a complete loss of the ability to mediate these chemical transformations. Restoration of the decarboxylating activity is obtained upon the addition of pyridoxal phosphate and iron(II1), but the addition of either singly causes no reconstitution. These studies show that the above reactions are mediated by a pyridoxal phosphate-dependent enzyme(s) and that a metallic ion is an obligatory requirement of the system.
last changed 2009/06/12 11:01

B6db references