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B6db references: hbnuah01

type Journal Article
authors Liu D., Liu X., Zhang L., Jiao H., Ju J., Zhao B.
title Biochemical characteristics of an alanine racemase from Aeromonas hydrophila HBNUAh01
journal Microbiology
sel selected
ui HBNUAh01
year (2015)
volume 84
number 2
pages 202209
keywords doi:10.1134/S0026261715020071
abstract We reveal that the genome of Aeromonas hydrophila HBNUAh01has an alanine racemase gene (alr-2); this gene encodes a functional enzyme that can complement the alanine racemase deficiency of Escherichia coli strain MB2795. The gene alr-2 was cloned and expressed in E. coli BL21 (DE3). The gene has an open reading frame (ORF) of 1230 bp encoding a protein of 369 amino acids with a calculated molecular mass of 40.39 kD. The amino acid sequence deduced revealed similarity of 98, 84 and 68% with alanine racemases from A. hydrophila ATCC7966, Aeromonas caviae_Ae398, Aeromonas vickers_b565, respectively. The optimal temperature and pH for enzyme activity was 37C and pH 10. The enzyme had broad substrate specificity. The alanine racemase was shown to belong to the pyridoxal 5′-phosphate (PLP)-dependent family of enzymes that require a certain concentration of PLP for activity. The kinetic parameters Km and Vmax at 40C of alanine racemase were 35.9 mM, 2898 units/mg for L-alanine and 15.36 mM, 1209 units/mg for D-alanine, respectively.
last changed 2017/07/12 13:02

B6db references